The Southern Research Molecular Biology team provides customized, high-quality services to commercial clients on an outsourced basis.

Our advanced capabilities include:

• qPCR assays and qRT-PCR assays
• Genome Walking, 5' and 3' RACE
• Expression analysis using oligonucleotide microarrays
• Identification and cloning of differentially expressed genes by suppressive subtractive hybridization
• Retroviral expression cloning
• Pathway screens using RNA interference strategies (siRNA) for knockdown of specific RNAs
• Pathway screens using inducible dominant negative mutants of signaling proteins
• Cloning and expression of genes of interest in heterologous bacterial systems
• Site-directed mutagenesis

Examples of qPCR/qRT-PCR assay-development projects include:

• qRT-PCR assays for various genes for correlative studies in Phase I/II trials
• qPCR assays to measure mitochondrial genomic DNA versus nuclear genomic DNA for assessing mitochondrial depletion
• qRT-PCR assays for assessing expression of genes encoding nuclear receptors, growth factors and their receptors, and their signaling intermediates